| 货号 | 9291L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F/E-P |
| 目标/特异性 | Phospho-Bad (Ser112) Antibody detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A), Bad (S112A/S136A) and treated with TPA or forskolin, using Phospho-Bad (Ser112) Antibody (upper) or Bad Antibody #9292 (lower). Western blot 免疫印迹分析分别转染了野生型Bad、Bad (Ser112A)、Bad (S136A)、Bad (S112A/S136A)并用TPA或者forskolin处理的 293 细胞的细胞提取液，所用抗体为Phospho-Bad (Ser112) Antibody (上图) 或者Bad Antibody #9292 (下图)研究。 | |
Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Bad Antibody #9292 (right) or Phospho-Bad (Ser112) Antibody (left). Western blot 免疫印迹分析未处理或者经TPA处理的COS 细胞的细胞提取液，使用抗体为 Bad Antibody #9292 (右图) 或者Phospho-Bad (Ser112) Antibody (左图)。 |
