| 货号 | 4539L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:25) IF-IC (1:25) |
| 供应商 | CST |
| 背景 | The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂，cdc2的激活受多个步骤的调控，包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而，在细胞进入有丝分裂的过程中，激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位，抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。 |
| 存放说明 | -20C |
| 计算分子量 | 34 |
Western blot analysis of nonphosphorylated GST-cdc2 fusion protein (lane 1), and extracts from SK-N-MC cells treated with hydroxyurea for 20 hours (lane 2), using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (upper), or cdc2 Antibody #9112 (lower).Western blot方法检测非磷酸化GST-cdc2融合蛋白（泳道1）和羟基脲处理20小时的SK-N-MC细胞提取物（泳道2），使用的抗体为Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (上图), 或 cdc2 Antibody #9112 (下图). | |
Western blot analysis of extracts from HeLa cells, untreated or hydroxyurea treated for 20 hours, using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb.western blot方法检测未处理和羟基脲处理20小时的HeLa细胞提取物，使用的抗体为Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb。 | |
Flow cytometric analysis of Jurkat cells, using Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb versus propidium iodide (DNA content).流式细胞术分析Jurkat细胞，使用的抗体为Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb ，PI染色法检测DNA含量。 | |
Confocal immunofluorescent analysis of asynchronous HeLa cells labeled with Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red).共聚焦免疫荧光方法分析非同步HeLa细胞，标记的抗体为Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb (绿色) 和 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (红色)。 |
