| 货号 | 9706L |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IF-F/IF-IC/F |
| 目标/特异性 | Phospho-Histone H3 (Ser10) (6G3) Mouse mAb detects endogenous levels of histone H3 only when phosphorylated at serine 10. The antibody does not cross-react with other phosphorylated histones or acetylated histone H3. |
| 使用方法 | WB(1:1000) F (1:25) IF-F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
使用Phospho-Histone H3 (Ser10) (6G3) Mouse mAb鼠单抗(上图)、Phospho-Histone H3 (Ser10) Antibody #9701 (中图)或Histone H3 Antibody #9712 (下图)，免疫印迹(Western blot)分析NIH/3T3细胞，细胞分为未处理 或处理了serum plus calyculin A (来引起磷酸化的H3)。 | |
Flow cytometric analysis of Phospho-Histone H3 (Ser10) (6G3) Mouse mAb staining of untreated (blue) or serum/calyculin-treated (green) Ramos cells compared to a nonspecific negative control antibody (red). 与一个非特异阴性control antibody (红色)比较，使用Phospho-Histone H3 (Ser10) (6G3) Mouse mAb鼠单抗进行流式细胞仪分析untreated (蓝色)或serum/calyculin-treated (绿色) Ramos细胞。 | |
Flow cytometric analysis of pacilitaxel-treated THP1 cells, using Phospho-Histone H3 (Ser10) (6G3) Mouse mAb versus propidium iodide (DNA content). The red population indicates positive Phospho-Histone H3 cells. |
