| 货号 | 3033L |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb detects NF-κB p65 only when phosphorylated at Ser536. It does not cross-react with the p50 subunit or other related proteins. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:1600) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
Flow cytometric analysis of HeLa cells, untreated (blue) or TNF-α-treated (green), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb compared to a nonspecific negative control antibody (red).流式细胞仪研究未经处理的HeLa细胞(蓝色)和经TNF-α处理的HeLa细胞(绿色)。所用抗体为Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb 并与非特异性的阴性对照抗体比对(红色)。 | |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TNF-α treated (#2169, 20 ng/ml for 5 minutes), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (upper) or NF-κB p65 Antibody #3034 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (上图) 或NF-κB p65 Antibody #3034 (下图)研究未经处理的和经TNF-α (#2169, 20 ng/ml, 5 min) 处理的HeLa和 NIH/3T3 细胞的细胞提取液。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) and TNF-α treated (#8902 at 20 ng/ml for 20 min, right), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® phalloidin 555 (red).共聚焦免疫荧光分析未经处理(左图)和经TNF-α(#8902 20 ng/ml,20 min, 右图)处理的HeLa细胞。所用的抗体为Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (绿色), 肌动蛋白用Alexa Fluor® phalloidin 555 (红色)标记。 | |
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (upper) and NF-κB p65 (C22B4) Rabbit mAb #4764 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (上图) 和 NF-κB p65 (C22B4) Rabbit mAb #4764 (下图) 研究经 TPA 分化24小时(#9905, 80 nM) 并用1 μg/ml LPS 处理一定时间的THP-1细胞的细胞提取液。 |
