| 货号 | 9216L |
| 反应种属 | Human/Mouse/Rat/Monkey/S.cerevisiae |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:2000) IP (1:50) F (1:1600) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin-treated (blue), using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb compared to a nonspecific negative control antibody (red)..p38 MAP激酶 (MAPK), 也被称为 RK (1)、CSBP (2)，是哺乳动物体内酵母HOG激酶的同源物，它参与信号传递级联反应，该级联反应能控制细胞对细胞因子和应激反应的应答(1-4)。p38 MAPK的四种异构体, p38α, β, γ (也被称为Erk6、SAPK3), 和δ (也被称为SAPK4)已经被鉴定出来。与SAPK/JNK途径相似，p38 MAPK由各种细胞应激反应激活，包括渗透压休克 、炎性反应、细胞因子、LPS、UV照射和生长因子。(1-5) MKK3, MKK6,SEK磷酸化p38 MAPK蛋白的苏氨酸（180位点）和酪氨酸（182位点）并使其活化。活化的p38 MAPK能将MAPKAP激酶磷酸化、活化 (3)，并磷酸化转录因子ATF-2(5), Max (6), and MEF2 (5-8)。SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole)是p38 MAPK的选择性抑制子。该复合物能通过p38 MAPK抑制MAPKAPK-2的活化和HSP27后续磷酸化 (9)。SB203580通过结合到ATP接合点上而抑制p38 MAPK的摧毁活性，但是不能通过上游激酶而抑制p38 MAPK的磷酸化(10)。 | |
Confocal immunofluorescent analysis of HeLa cells either untreated (left) or anisomycin treated (right) labeled with Phospho-p38 MAP Kinase (Thr180/Tyr182) (28B10) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor Phalloidin 555 (red)..p38 MAP激酶 (MAPK), 也被称为 RK (1)、CSBP (2)，是哺乳动物体内酵母HOG激酶的同源物，它参与信号传递级联反应，该级联反应能控制细胞对细胞因子和应激反应的应答(1-4)。p38 MAPK的四种异构体, p38α, β, γ (也被称为Erk6、SAPK3), 和δ (也被称为SAPK4)已经被鉴定出来。与SAPK/JNK途径相似，p38 MAPK由各种细胞应激反应激活，包括渗透压休克 、炎性反应、细胞因子、LPS、UV照射和生长因子。(1-5) MKK3, MKK6,SEK磷酸化p38 MAPK蛋白的苏氨酸（180位点）和酪氨酸（182位点）并使其活化。活化的p38 MAPK能将MAPKAP激酶磷酸化、活化 (3)，并磷酸化转录因子ATF-2(5), Max (6), and MEF2 (5-8)。SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole)是p38 MAPK的选择性抑制子。该复合物能通过p38 MAPK抑制MAPKAPK-2的活化和HSP27后续磷酸化 (9)。SB203580通过结合到ATP接合点上而抑制p38 MAPK的摧毁活性，但是不能通过上游激酶而抑制p38 MAPK的磷酸化(10)。 | |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated as indicated, using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (upper) or p38 MAPK Antibody #9212 (lower).western blot方法检测细胞提取物：未处理和按规定处理的HeLa 、NIH/3T3细胞，使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (上图) 和p38 MAPK Antibody #9212 (下图)。 |
