| 货号 | 9138L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/F |
| 目标/特异性 | Phospho-Stat3 (Tyr705) (3E2) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. The antibody does not significantly cross-react with other members of the Stat family. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 79, 86 |
Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha-treated (100 ng/ml), using Phospho-Stat3 (Tyr705) (3E2) Mouse mAb.Western免疫印迹。用Phospho-Stat3 (Tyr705) (3E2) Mouse mAb研究未经处理的或经过 IFN-a (100 ng/ml)处理的HeLa细胞的细胞提取液。 | |
Flow cytometric analysis of NIH/3T3 cells, untreated (red) or IFN-alpha treated (blue), using Phospho-Stat3 (Tyr705) (3E2) Mouse mAb.流式细胞仪研究未经处理的NIH/3T3 细胞(红色)和经IFN-α处理的NIH/3T3 细胞(蓝色)。所用抗体为Phospho-Stat3 (Tyr705) (3E2) Mouse mAb。 |
