| 货号 | 2966L |
| 反应种属 | Human/Mouse/Rat/Hamster |
| 来源宿主 | Mouse |
| 应用 | IP/IF-IC/F |
| 目标/特异性 | Akt (5G3) Mouse mAb detects endogenous levels of Akt. This antibody does not cross-react with other related proteins. |
| 使用方法 | IP(1:100) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Immunoprecipitation of Akt from PDGF (100 ng/ml for 11 minutes) treated and untreated NIH/3T3 cell lysates, using Akt (5G3) Mouse mAb (left) and control Akt Antibody #9272 (right). Western blot detection was performed using Akt Antibody #9272. Immunoprecipitation得到PDGF (100 ng/ml )处理11分钟或未处理的NIH/3T3细胞提取物中的Akt蛋白，使用的抗体 Akt (5G3) Mouse mAb 鼠单抗（左图) 和 control Akt Antibody #9272 (右图)。Western blot 方法检测使用的抗体是Akt Antibody #9272。 | |
Akt (5G3) Mouse mAb #2966 staining of untreated (blue) or LY294002-treated (green) jurkat cells compared to a nonspecific negative control antibody (red). |
