Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (PE Conjugate)
货号：
5315S 基本售价：
4460.0 元 规格：
100ul
产品信息
概述| 货号 | 5315S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060. |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/D.melanogaster/Zebrafish/Bovine |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of Akt only when phosphorylated at Ser473. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 (PI3 Kinase Inhibitor) #9901, Wortmannin (PI3 Kinase Inhibitor) #9551 and U0126 (MEK1/2 Inhibitor) #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (PE Conjugate). 流式细胞术分析Jurkat 细胞，分未处理组和 (绿色)或采用LY294002 (PI3K抑制剂) #9901, Wortman-nin (PI3K抑制剂) #9551 和 U0126 (MEK1/2 抑制剂) #9903 (蓝色)处理组，所用抗体为Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb兔单抗 (PE Conjugate). |
