Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (PE Conjugate)
货号：
8466S 基本售价：
4460.0 元 规格：
100ul
产品信息
概述| 货号 | 8466S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558. |
| 反应种属 | Human/Mouse/Rat/Hamster |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb detects endogenous levels of GSK-3β only when phosphorylated at Ser9. This antibody reacts with denatured components of bovine serum, including BSA. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of NIH/3T3 cells treated with hPDGF-BB #8912 and λ phosphatase (red), untreated (blue), or treated with hPDGF-BB #8912 only (green) using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (PE Conjugate). 流式细胞术分析用hPDGF-BB #8912和λ 磷酸酶 (红色)处理的NIH/3T3细胞，未刺激组为蓝色， hPDGF-BB #8912 处理组为绿色，所用抗体为Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb兔单抗 (PE Conjugate)。 |
