| 货号 | 3642S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 reacts with phospho-histone H3 (Ser10) from human, mouse, rat, and monkey. CST expects that Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated) will also recognize phospho-histone H3 (Ser10) in these species. |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F/IF-IC/F |
| 目标/特异性 | Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated) detects endogenous levels of histone H3 only when phosphorylated at Ser10. |
| 使用方法 | WB(1:1000) F (1:400) IF-F () IF-IC (1:400) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Western blot analysis of extracts from HeLa cells, untreated or paclitaxel-treated, using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated). 使用Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated)，免疫印迹分析untreated或paclitaxel-treated HeLa细胞。 | |
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated) versus DRAQ5® (DNA content). 使用Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated)和DRAQ5® (DNA含量)染色，流式细胞仪分析untreated Jurkat细胞。 | |
Confocal immunofluorescence analysis of P14 rat cerebellum using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated) (green), GFAP (GA5) Mouse mAb #3670 (red) and Doublecortin Antibody #4604 (blue). 使用Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Biotinylated) (绿色)、GFAP (GA5) Mouse mAb #3670 (红色)和Doublecortin Antibody #4604 (蓝色)，免疫组化分析P14 rat cerebellum细胞。 |
