| 货号 | 6992S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb #5482. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of NDRG1 when phosphorylated at Thr346. This antibody likely cross-reacts with other conserved phosporylation sites on NDRG1 at positions Thr356 and Thr366. |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3. |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of C2C12 cells, insulin-treated (left) or treated with LY294002 #9901 (right) using Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 胰岛素处理（左）或LY294002#9901（右）处理后的C2C12细胞，使用Phospho-NDRG1 (Thr346) (D98G11) XP® 兔单抗（Alexa Fluor®488偶联）（绿色）进行激光共聚焦荧光分析。使用DY-554phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5®#4084（DNA荧光染料） | |
