| 货号 | 8957S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #8516.Cell Signaling Technology公司抗体偶联Alexa Fluor® 555荧光素染料并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联Alexa Fluor® 488的Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit mAb#8516显示同样的物种交叉反应. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 555 |
| 背景 | The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).成视网膜细胞瘤抑制蛋白，Rb，通过控制G1期的细胞限制点以调控细胞增殖（1）。Rb有3个功能迥异的结构域，与一系列重要的调控蛋白相结合，包括E2F家族调控因子，c-Abl酪氨酸激酶以及带有LXCXE保守结构域的蛋白（2-4）。CDK引起的细胞周期依赖性磷酸化会抑制Rb与靶蛋白结合从而促进细胞周期的进行（5）。细胞周期蛋白 D-CDK4/6起始的磷酸化，伴随着细胞周期蛋白E-CDK2的磷酸化，似乎能够失活Rb并引发细胞周期（6）。体外实验已经检测到有不同的细胞周期蛋白依赖性激酶/细胞周期蛋白复合物（6-8）同时体内试验证明细胞周期蛋白D1对于780位丝氨酸的磷酸化是必需的（9）。 |
| 存放说明 | 4C |
Flow cytometic analysis of BT-549 (blue) and Jurkat (green) cells using Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate).BT-549(蓝色)和Jurkat（绿色）使用Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit mAb（偶联Alexa Fluor® 555）进行流式细胞分析。 | |
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or λ phosphatase-treated (middle), and BT-549 cells (right), using Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) (red). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (green).未处理的MCF7（左），λ phosphatase处理的MCF7（中）和BT-549细胞（右）使用Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit mAb（偶联Alexa Fluor® 555）进行激光共聚焦免疫荧光分析。肌动蛋白丝使用Alexa Fluor® 488鬼笔环肽标记（绿色）。 |
