Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)【科瑞奥博】

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Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)

货号： 4803S 基本售价： 4460.0 元规格： 100ul

产品信息

概述
货号	4803S
描述	This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858.
反应种属	Human/Mouse/Rat/Monkey/Mink/S.cerevisiae
来源宿主	Rabbit
应用	IF-IC/F
目标/特异性	Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of S6 ribosomal protein only when phosphorylated at Ser235 and Ser236.
使用方法	F(1:50) IF-IC (1:100)

性能
供应商	CST
标记	Alexa Fluor 488
背景	One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
存放说明	4C

参考图片

Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002/wortmannin/U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate).

使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 (Alexa Fluor® 488 接合)，流式细胞术分析Jurkat细胞，细胞分为未处理组（绿色）和LY294002/Wortmannin/U0126处理组（蓝色）。

Confocal immunofluorescent analysis of HeLa cells, treated with wortmannin and U0126 (left) or PMA (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 (Alexa Fluor® 488接合)（绿色），共聚焦免疫荧光观察磷酸化S6核糖体蛋白在HeLa细胞中定位，分为wortmannin和U0126处理组（左图）和PMA处理组（右图）。蓝色伪彩= DRAQ5™ (DNA荧光染料)。