Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Biotinylated)
货号：
3945S 基本售价：
4460.0 元 规格：
100ul
产品信息
概述| 货号 | 3945S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested in-house by Western Blot and flow cytometric analysis of human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858). |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 标记 | Biotin |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
参考图片Western Blot analysis of extracts from HeLa cells, either untreated or treated with insulin alone or insulin and phosphatase, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Biotinylated). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 (生物素标记)，免疫印迹（Western Blot）分析HeLa细胞，分为对照组，胰岛素单独处理或胰岛素加磷酸酶组。 |
