Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Pacific Blue™ Conjugate)
货号：
8520S 基本售价：
4460.0 元 规格：
100ul
产品信息
概述| 货号 | 8520S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858. |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and Ser236. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Pacific Blue |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Pacific Blue™ Conjugate). 流式细胞术分别检测未处理（绿色）或经LY294002 #9901, Wortmannin #9951, 以及U0126 #9903处理（蓝色）的Jurkat细胞活性，使用的抗体为Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 (Pacific Blue™ Conjugate)。 |
