Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (PE Conjugate)
货号：
5316S 基本售价：
4460.0 元 规格：
100ul
产品信息
概述| 货号 | 5316S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858. |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (PE conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 (PI3 Kinase Inhibitor) #9901, Wortmannin (PI3 Kinase Inhibitor) #9551 and U0126 (MEK1/2 Inhibitor) #9903 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (PE Conjugate). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 (PE接合)，流式细胞术分析Jurkat细胞，细胞分为未处理组（绿色）和LY294002 (PI3 Kinase抑制剂) #9901、Wortmannin (PI3 Kinase抑制剂) #9551 和U0126 (MEK1/2抑制剂) #9903处理组（蓝色）。 |
