| 货号 | 9468S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human and mouse cells, respectively. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 594 |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of C2C12 cells, insulin-treated (left) or treated with LY294002 #9901, Rapamycin #9904, and U0126 #9903 (right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 采用共聚焦免疫荧光术分别检测insulin处理（左图）或LY294002 #9901, Wortmannin #9951, 以及U0126 #9903处理（右图）的C2C12细胞，使用的抗体为Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗 (Alexa Fluor® 594 接合) (红色)。肌动蛋白微丝使用Alexa Fluor® 488 phalloidin进行标记（绿色）。蓝色假彩色= DRAQ5® #4084 (荧光DNA染料)。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate). 流式细胞术分别检测未处理（绿色）或经LY294002 #9901, Wortmannin #9951, 以及U0126 #9903处理（蓝色）的Jurkat细胞，使用的抗体为Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗 (Alexa Fluor® 594 接合)。 |
