| 货号 | 5558S |
| 反应种属 | Human/Mouse/Rat/Hamster |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb detects endogenous levels of GSK-3β only when phosphorylated at Ser9. This antibody reacts with denatured components of bovine serum, including BSA. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | -20C |
| 计算分子量 | 46 |
Confocal immunofluorescent analysis of wild type mouse embryonic fibroblasts (MEFs) (top row), GSK-3β (-/-) MEFs (middle row) , or PC-3 cells (bottom row), untreated (left), LY294002- and Wortmannin-treated (#9901 and #9951 respectively; center) or lambda phosphatase-treated (right), using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). | |
共聚焦免疫荧光分析野生型小鼠胚胎成纤维细胞 (MEFs) (top row), GSK-3β (-/-) MEFs (middle row) , or PC-3 cells (bottom row), 未处理组 (左), LY294002- 和 Wortmannin处理组 (#9901 and #9951 respectively; 中) or λ 磷酸酶处理 (右)，所用抗体为Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (绿色).蓝色伪彩是DRAQ5 ® #4084 (荧光 DNA 染料). ). (MEF wild type, GSK-3α (-/-) 和GSK-3β (-/-) 细胞是由加拿大多伦多大学Dr. Jim Woodgett友情提供). | |
