| 货号 | 3377S |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb detects endogenous levels of histone H3 only when phosphorylated at Ser10. The antibody does not cross-react with other phosphorylated histones or with acetylated histones. |
| 使用方法 | WB(1:1000) F (1:1600) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb versus propidium iodide (DNA content). The boxed population indicates Phospho-Histone H3 (Ser10) positive cells. 使用Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb兔单抗与propidium iodide (DNA 含量)，流式细胞仪分析Jurkat细胞。方框群组是阳性Phospho-Histone H3 (Ser10)细胞。 | |
Confocal immunofluorescent analysis of HeLa cells using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb兔单抗 (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。蓝色= DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from HeLa cells, either untreated or treated with nocodazole (0.1 mg/ml for 18 hours), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 Antibody #9715 (lower). Phospho-specificity of the antibody is shown by further treatment of the lysate with λ phosphatase. 使用Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377兔单抗 (上图)或Histone H3 Antibody #9715 (下图)，免疫印迹(Western blot)分析HeLa细胞，细胞分为untreated 或处理了nocodazole (0.1 mg/ml for 18 hours)。抗体的磷酸化特异性通过λ phosphatase进一步处理裂解物从而证明。 | |
Peptide dot blot analysis demonstrating Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb antibody specificity. Antibody binding to pre-coated histone H3 peptides is shown using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb #53348, |
