| 货号 | 8722S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb recognizes endogenous levels of MARCKS protein only when phosphorylated at Ser167 and Ser170. This antibody may also detect MARCKS mono-phosphorylated at Ser167. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5). |
| 存放说明 | -20C |
| 计算分子量 | 75 rodent, 80 human |
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left), treated with TPA #4174 (200 nM, 15 min; middle) or treated with TPA #4174 and λ phosphatase (right), using Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测血清饥饿处理的HeLa细胞 (左图)，细胞用TPA #4174 (200 nM, 15 min; 中图) 或TPA #4174和λ 磷酸酶处理(右图)，使用的抗体为 Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb (绿色). 蓝色伪彩色为DNA荧光染料 (产品信息为DRAQ5® #4084). | |
Western blot analysis of extracts from various cell lines, serum-starved overnight and then untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MARCKS (Ser167/Ser170) (D13E4) XP® Rabbit mAb. Western blot方法检测多个细胞系的提取物，细胞血清饥饿过夜，随后不处理（-）或TPA #4174 (200 nM, 15 min; +)处理，使用的抗体为Phospho-MARCKS (Ser167/Ser170) (D13E4) XP® Rabbit mAb. | |
Western blot analysis of extracts from serum-starved SH-SY5Y and SK-N-MC cells, untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb (upper) and MARCKS (D88D11) XP® Rabbit mAb #5607 (lower). Western blot方法检测血清饥饿处理的SH-SY5Y和SK-N-MC细胞的提取物，细胞不处理（-）或经TPA #4174 (200 nM, 15 min; +)处理，使用的抗体为Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb (上图)和MARCKS (D88D11) XP® Rabbit mAb #5607 (下图). | |
Flow cytometric analysis of HeLa cells, serum starved (blue) or serum starved and treated with TPA #4174 (200nM, 15 min; green), using Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary Ab. 流式分析Hela细胞，血清饥饿（蓝色）或血清饥饿后使用TPA#4174处理（200nM，15分钟；绿色），使用Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414为二抗. |
