Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb【科瑞奥博】

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Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb

货号： 12958S 基本售价： 4280.0 元规格： 100ul

产品信息

概述
货号	12958S
反应种属	Human/Mouse/Rat/Hamster/MonkeyDog
来源宿主	Rabbit
应用	W/IP/IF-IC/F
使用方法	WB(1:1000) IP (1:50) F (1:200) IF-IC (1:100)

性能
供应商	CST
存放说明	-20C
计算分子量	125

参考图片

Flow cytometric analysis of Jurkat cells using Phospho-MCM2 (Ser139) (D1Z8X) XP^® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 (DNA content). Anti-rabbit IgG (H+L), F(ab)₂ Fragment (Alexa Fluor^® 488 conjugate) #4412 was used as a secondary Ab.使用Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb和碘化丙啶PI)/RNase Staining Solution #4087 (DNA content)对Jurkat细胞进行流式分析。Anti-rabbit IgG (H+L), F(ab)2Fragment (Alexa Fluor® 488 conjugate) #4412作为二抗。

Confocal immunofluorescent analysis of WI-38 cells, serum-deprived (48 hr; upper left), or serum-treated (10%, 24 hr; upper right) and HeLa cells, untreated (lower left), or λ phosphatase-treated (2 hr; lower right), using Phospho-MCM2 (Ser139) (D1Z8X) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).血清饥饿(48 hr; 左上)或血清处理(10%, 24 hr; 右上)的WI-38细胞，未处理（左下）和λ phosphatase-treated (2 hr; 右下)的HeLa细胞，使用Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb（绿色）进行激光共聚焦免疫荧光分析。

Western blot analysis of HeLa cell extracts, untreated (-) or calf intestinal phosphatase (CIP) and λ phosphatase-treated (+), and extracts of T98G cells serum-starved for 48 hours (-) or serum starved for 48 hours followed by serum-stimulation for 24 hours (+), using Phospho-MCM2 (Ser139) (D1Z8X) XP^® Rabbit mAb (upper), MCM2 (D7G11) XP^® Rabbit mAb #3619 or α-Actinin (D6F6) XP^® Rabbit mAb #6487.未处理（-）或牛小肠碱性磷酸酶（CIP）和λ phosphatase处理（+）的HeLa细胞提取物，以及血清饥饿48小时（-）或血清饥饿48小时或再用血清刺激24小时（+）的T98G细胞提取物，使用Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb (上), MCM2 (D7G11) XP® Rabbit mAb #3619 or α-Actinin (D6F6) XP® Rabbit mAb #6487（下）进行western blot分析。

Immunoprecipitation of phospho-MCM2 (Ser139) from T98G cell extracts, using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or Phospho-MCM2 (Ser139) (D1Z8X) XP^® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-MCM2 (Ser139) (D1Z8X) XP^® Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb (lane 3)对T98G细胞提取物中获得的磷酸化MCM2（Ser139）进行免疫沉淀实验。Lane1是10%上样量。使用Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb进行western blot分析。