Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb【科瑞奥博】

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Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb

货号： 5025S 基本售价： 4280.0 元规格： 100ul

产品信息

概述
货号	5025S
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb detects endogenous levels of RelB only when phosphorylated at Ser552.
使用方法	WB(1:1000) IP (1:100) F (1:800) IF-IC (1:400)

性能
供应商	CST
背景	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
存放说明	-20C
计算分子量	70

参考图片

Immunofluorescent analysis of Raji cells, treated with TPA #4174 (left) or serum-starved (right) using Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb (green). Red = Propidium Iodide (fluorescent DNA dye).免疫荧光分析经TPA #4174 (左图)或血清饥饿(右图)的Raji细胞。所用抗体为Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb (绿色)。Red = Propidium Iodide (DNA荧光染料 )。

Flow cytometric analysis of Raji cells, untreated (blue) or treated with TPA #4174 (green), using Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb.流式细胞仪分析未经处理(蓝色) 或经TPA #4174 (绿色)处理的Raji细胞。所用抗体为Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb。

Western blot analysis of extracts from A20 and CTTL2 cells, untreated or treated with TPA #4174 (200nM, 30 minutes) alone or with λ phosphatase, using Phospho-RelB (Ser552) (D41B9) Rabbit mAb.Western免疫印迹。用Phospho-RelB (Ser552) (D41B9) Rabbit mAb研究未经处理的和经TPA #4174 (200nM, 30 min)处理或经TPA #4174 (200nM, 30 min) 和λ phosphatase共处理的A20和CTTL2 细胞的细胞提取液。