| 货号 | 4322S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-Stat5 (Tyr694) (D47E7) XPTM Rabbit mAb detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml for 5 min), TF-1 cells, untreated or treated with Human Granulocyte Macrophage Colony Stimulating Factor #8922 (hGM-CSF; 100ng/ml for 10 min), and NK-92 cells, untreated or treated with Human Interleukin-2 #8907 (hIL-2; 100ng/ml for 10 min), using Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb (upper) or total Stat5 (3H7) Rabbit mAb #9358 (lower).Western免疫印迹。用Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb (上图) 或total Stat5 (3H7) Rabbit mAb #9358 (下图) 研究未经处理的和经erythropoietin (EPO; 3 units/ml,5 min)处理的UT-7细胞的细胞提取液;未经处理的和经Human Granulocyte Macrophage Colony Stimulating Factor #8922 (hGM-CSF; 100ng/ml ,10 min)处理的TF-1细胞的细胞提取液;未经处理的和经Human Interleukin-2 #8907 (hIL-2; 100ng/ml,10 min)处理的NK-92 细胞的细胞提取液。 | |
Flow cytometric analysis of TF-1 cells, untreated (blue) or GM-CSF treated (green), using Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb.流式细胞仪研究未经处理的TF-1细胞(蓝色)和经GM-CSF 处理的TF-1细胞(绿色)。所用抗体为Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of A-431 cells, EGF-treated (left) or untreated (right), using Phospho-Stat5 (Tyr694) XP®(D47E7) Rabbit mAb (green) and Pan-Keratin (C11) Mouse mAb #4545 (red).共聚焦免疫荧光分析经EGF处理的A-431细胞(左图)或者未经处理的A-431细胞 (右图)，所用抗体为Phospho-Stat5 (Tyr694) XP®(D47E7) Rabbit mAb (绿色) 和 Pan-Keratin (C11) Mouse mAb #4545 (红色)。 |
