Vimentin (D21H3) XP® Rabbit mAb (PE Conjugate)
货号：
12020S 基本售价：
4264.0 元 规格：
100ul
产品信息
概述| 货号 | 12020S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Vimentin (D21H3) XP® Rabbit mAb #5741. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Vimentin (D21H3) XP® Rabbit mAb (PE Conjugate) detects endogenous levels of total vimentin protein. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell-specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentins dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of MCF7 (blue) and HeLa (green) cells using Vimentin (D21H3) XP® Rabbit mAb (PE Conjugate).使用Vimentin (D21H3) XP® Rabbit mAb（偶联PE）对MCF7（蓝色）和HeLa（绿色）进行流式分析。 |
