| 货号 | 5588S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGF Receptor (D38B1) XP® Rabbit mAb #4267.该CST公司生产的抗体与Alexa Fluor® 647荧光染料偶联并在人的细胞中进行了免疫荧光检测。该抗体具有和未偶联的抗EGFR的兔源单克隆抗体(D38B1) XP® #4267相同的物种交叉反应特性。 |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total EGF receptor protein. The antibody does not cross-react with other proteins of the ErbB family. |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat cells (blue) and HeLa cells (green) using EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).用抗EGFR的兔源单克隆抗体(D38B1) XP®(偶联Alexa Fluor® 647) 对Jurkat细胞 (蓝色)和 HeLa细胞(绿色)进行免疫荧光检测。 | |
Confocal immunofluorescent analysis of A549 cells, serum-starved (left) or EGF-treated (#8916, right), using EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #9683 (green).用抗EGFR的兔源单克隆抗体(D38B1) XP®(偶联Alexa Fluor® 647) （蓝色-伪色）和抗Acetyl-Histone H3 (Lys9) (C5B11)的兔源单克隆抗体(偶联Alexa Fluor® 488 ) #9683 (绿色)对经过血清饥饿（左）或经过EGF处理的(#8916, 右)A549细胞进行免疫荧光共聚焦检测。 |
