| 货号 | 12230S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Histone H3 (D1H2) XP® Rabbit mAb #4499. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | Histone H3 (D1H2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total histone H3 protein. This antibody does not cross-react with other histones. |
| 使用方法 | F(1:50) IF-IC (1:3200) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of HeLa cells using Histone H3 (D1H2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with DY-554 phalloidin (red).采用共聚焦免疫荧光术检测Hela细胞，使用的抗体为Histone H3 (D1H2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (蓝色，假彩色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。 | |
Human whole blood was fixed, lysed, and permeabilized as per the Cell Signaling Technology Alternate Flow Protocol and stained using Histone H3 (D1H2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate). The forward/side-scatter lymphocyte gate was applied to a histogram depicting the mean fluorescence intensity of Histone H3 (blue) versus that of concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (red).按照CST 流式替代操作方法对人全血进行固定，溶解以及透性化处理，使用Histone H3 (D1H2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)进行染色。采用前向角/侧向角散射光设置淋巴细胞门，以直方图来表示Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (蓝色)和与之相对的浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (红色) 的平均荧光强度。 |
