| 货号 | 4040S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same cross-reactivity as the unconjugated antibody (Jak2 (D2E12) XP® Rabbit mAb #3230). |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members. |
| 使用方法 | WB(1:1000) F (1:100) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. |
| 存放说明 | -20C |
| 计算分子量 | 125 |
Western blot analysis of extracts from various cell lines using Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.Western免疫印迹。用Jak2 (D2E12) XP® Rabbit mAb (Biotinylated)研究各种细胞系的细胞提取液。用Streptavidin-HRP #3999检测。 | |
Flow cytometric analysis of UT-7 cells using Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096 (red).流式细胞仪研究UT-7 细胞，所用抗体为Jak2 (D2E12) XP® Rabbit mAb (Biotinylated) (蓝色) 与Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096 (红色)对比。 |
