LC3B (D11) XP® Rabbit mAb (PE Conjugate)
货号：
8899S 基本售价：
4264.0 元 规格：
100ul
产品信息
概述| 货号 | 8899S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated LC3B (D11) XP® Rabbit mAb #3868. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | LC3B (D11) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total LC3B protein. Cross-reactivity may occur with other LC3 isoforms. Stronger reactivity is observed with the type II form of LC3B. Weaker reactivity is observed with rodent LC3B. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of ACHN cells, untreated (blue) or chloroquine-treated (green), using LC3B (D11) XP® Rabbit mAb (PE Conjugate). 流式细胞术分析ACHN细胞，未处理（蓝色）或chloroquine 处理（绿色），使用的抗体是 LC3B (D11) XP® Rabbit mAb 兔单抗（PE结合）。 |
