NF-κB p65 (D14E12) XP® Rabbit mAb (PE Conjugate)
货号：
9609S 基本售价：
4264.0 元 规格：
100ul
产品信息
概述| 货号 | 9609S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated NF-κB p65 (D14E12) XP® Rabbit mAb #8242. |
| 反应种属 | Human/Mouse/Rat/Hamster/MonkeyDog |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | NF-κB p65 (D14E12) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total NF-κB p65/RelA protein. This antibody does not cross react with other NF-κB/Rel family members. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HeLa cells using NF-κB p65 (D14E12) XP® Rabbit mAb (PE Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (red).流式细胞术分析HeLa细胞，使用抗体是NF-κB p65 (D14E12) XP® Rabbit mAb (PE Conjugate) (绿色)以及相对应浓度的Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (红色)。 |
