| 货号 | 40969S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | NRF2 (D1Z9C) XP®Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total NRF2 protein. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintain cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5). |
| 存放说明 | 4C |
| 参考文献 | 1 . Cullinan, S.B. et al. (2004) Mol Cell Biol 24, 8477-86. 2 . Nguyen, T. et al. (2005) J Biol Chem 280, 32485-92. 3 . Jaiswal, A.K. (2004) Free Radic Biol Med 36, 1199-207. 4 . Suzuki, M. et al. (2008) Am J Respir Cell Mol Biol 39, 673-82. 5 . Homma, S. et al. (2009) Clin Cancer Res 15, 3423-32. |
Flow cytometric analysis of MEF cells, untreated (blue) or treated with MG-132 #2194 (10 μM, 10 hr; green), using NRF2 (D1Z9C) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate). |
