| 货号 | 83324S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | Syk (D3Z1E) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Syk protein. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9). |
| 存放说明 | 4C |
| 参考文献 | 1 . Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33. 2 . Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18. 3 . Chu, D.H. et al. (1998) Immunol Rev 165, 167-80. 4 . Turner, M. et al. (2000) Immunol Today 21, 148-54. 5 . Coopman, P.J. et al. (2000) Nature 406, 742-7. 6 . Deckert, M. et al. (1998) J Biol Chem 273, 8867-74. 7 . Rao, N. et al. (2001) EMBO J 20, 7085-95. 8 . Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15. 9 . Zhang, J. et al. (2000) J Biol Chem 275, 35442-7. |
Flow cytometric analysis of RL-7 cells using Syk (D3Z1E) Rabbit mAb (PE Conjugate) (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #5742 (red). |
