| 货号 | 9669S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175) Antibody #9661. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate). 流式细胞术检测 Jurkat 细胞，未处理(蓝色) or etoposide（依托泊苷）处理 (绿色), 使用抗体为 Cleaved Caspase-3 (Asp175) Antibody (耦合Alexa Fluor®488). | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or staurosporine-treated (right), using Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye). 共聚焦免疫荧光图片显示 HeLa 细胞，未处理（左）或 staurosporine（星胞菌素）处理（右）用 Cleaved Caspase-3 (Asp175) Antibody (偶联Alexa Fluor® 488 ) （绿色）。微丝用Alexa Fluor® 555 phalloidin （鬼比环肽）（红色）。蓝色伪彩=DRAQ5® #4084 （DNA荧光染料）。 |
