| 货号 | 3624S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Tubulin (9F3) Rabbit mAb #2128. |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish/Bovine |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total β-tubulin protein and does not cross-react with recombinant α-tubulin. |
| 使用方法 | F(1:50) IF-IC (1:250) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | 4C |
Flow cytometric analysis of untreated NIH/3T3 cells using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) (green) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (red).与XP® Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (红色)比较，使用Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) (绿色)标记，流式细胞仪分NIH/3T3细胞。 | |
Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Red = Propidium Iodide (fluorescent DNA dye).使用β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) (蓝色)标记，共聚焦免疫荧光分析HeLa细胞。红色= Propidium Iodide (DNA荧光染料)。 |
