| 货号 | 5057S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (β-Actin (13E5) Rabbit mAb #4970).Cell Signaling Technology(CST)抗体在最佳条件下连接着biotin。该抗体被期望像非连接的抗体(β-Actin (13E5) Rabbit mAb #4970)一样拥有同一物种之间交叉反应活性。 |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 使用方法 | WB(1:1000) F (1:200) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6).Actin是一个广泛存在的真核细胞蛋白质，其实细胞骨架的重要组成成分。在哺乳类动物中至少有六种亚型。非肌肉组织β-和γ-actin也称作细胞质actin，它们在非肌肉组织中主要的表达，目的是控制细胞结构和运动(1)。α-cardiac和α-skeletal actin分别在心脏横纹肌和骨骼肌表达；两个平滑肌actins（α-和γ-actin）各自主要地发现在血管平滑肌和肠道平滑肌。这些actin亚型调节肌肉细胞的收缩潜能(1)。Actin主要存在作为一个纤维聚合物F-actin。在一些进程例如细胞分裂、内吞作用或应激中回应细胞骨架重组信号，cofilin促进分裂和F-actin的解聚，从而导致在单体球状形式G-actin的增加(2)。Arp2/3复合物使F-actin片段稳定，并且促进新的actin细丝形成(2)。有研究报道actin是在主要乳腺肿瘤中高度磷酸化(3)。在凋亡条件下actin的分裂已经在体外、心肌和骨骼肌中观察(4-6)。通过caspase-3剪切的Actin可能加速泛素/蛋白酶体依赖的肌肉蛋白水解(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from various cell lines using β-Actin (13E5) Rabbit mAb (Biotinylated).使用β-Actin (13E5) Rabbit mAb (Biotinylated)，免疫印迹（Western Blot）分析不同细胞系β-Actin蛋白水平。 | |
Flow cytometric analysis of HeLa cells using β-Actin (13E5) Rabbit mAb (Biotinylated) (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096 (red).与Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096 (红色)比较，使用β-Actin (13E5) Rabbit mAb (Biotinylated)（蓝色）标记，流式细胞仪分析HeLa细胞。 |
