| 货号 | 8584S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human and monkey cells, respectively. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Actin (13E5) Rabbit mAb #4970. |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms. |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.使用β-Actin (13E5)Rabbit mAb（偶联Alexa Fluor® 647）（蓝色假色）对COS-7细胞进行激光共聚焦免疫荧光分析。红色=碘化丙啶(PI)/RNase Staining Solution #4087. | |
Flow cytometric analysis of HeLa cells using β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (red).使用β-Actin (13E5)Rabbit mAb（偶联Alexa Fluor® 647）（绿色）对比相应浓度的Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (红色)对HeLa细胞进行流式分析。 |
