α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate)【科瑞奥博】

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α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate)

货号： 8058S 基本售价： 4060.0 元规格： 100ul

产品信息

概述
货号	8058S
描述	This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (DM1A) Mouse mAb #3873.
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Mouse
应用	IF-IC/F
目标/特异性	α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total α-tubulin protein.
使用方法	F(1:50) IF-IC (1:100)

性能
供应商	CST
标记	Alexa Fluor 488
背景	The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
存放说明	4C

参考图片

Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。

Flow cytometric analysis of Jurkat cells, using α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).

与Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (红色)比较，使用α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (蓝色)标记，流式细胞仪分Jurkat细胞。