Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Pacific Blue™ Conjugate)
货号：
11857S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 11857S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649. |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish/C.elegans |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of histone H3 only when acetylated at Lys9. This antibody does not cross-react with other acetylated histones. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Pacific Blue |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HeLa cells, untreated (blue) or SAHA-treated (green), using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Pacific Blue™ Conjugate).流式细胞术检测未处理（蓝色）和经SAHA处理过（绿色）的HeLa细胞提取物，使用的抗体为Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Pacific Blue™ Conjugate)。 |
