| 货号 | 2944S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #2966 reacts with human, mouse, rat and hamster Akt protein. CST expects that Akt (5G3) Mouse mAb (Alexa Fluor® 647 Conjugate) will also recognize Akt in these species. |
| 反应种属 | Human/Mouse/Rat/Hamster |
| 来源宿主 | Mouse |
| 应用 | F |
| 目标/特异性 | Akt (5G3) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of Akt. This antibody does not cross-react with other related proteins. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat cells, untreated (left) or or treated with LY294002, wortmannin and U0126 (right), using Akt (5G3) Mouse mAb (Alexa Fluor® 647 Conjugate) and Phospho-Akt (Ser473) (D9E) Rabbit mAb (Alexa Fluor® 488Conjugate) #4071. 流式细胞方法分析Jurkat 细胞，左图是未处理的，右图是用 LY294002, wortmannin 和 U0126处理的； 使用的抗体是 Akt (5G3) Mouse mAb 鼠单抗(Alexa Fluor® 647 Conjugate) 和 Phospho-Akt (Ser473) (D9E) Rabbit mAb兔单抗 (Alexa Fluor® 488Conjugate) #4071。 | |
Flow cytometric analysis of untreated Jurkat cells (green) using Akt (5G3) Mouse mAb (Alexa Fluor® 647 Conjugate) compared to a nonspecific negative control antibody (red). 流式细胞方法分析Jurkat 细胞，绿色是未处理的使用抗体是Akt (5G3) Mouse mAb 鼠单抗(Alexa Fluor® 647 Conjugate)；红色是非特异的阴性对照抗体。 |
