| 货号 | 4821S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The antibody exhibits the same species cross-reactivity as the unconjugated Akt (pan) (40D4) Mouse mAb #2920. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/F |
| 目标/特异性 | Akt (pan) (40D4) Mouse mAb (Biotinylated) detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from various cell lines using Akt (pan) (40D4) Mouse mAb (Biotinylated). Western blot分析各种细胞的提取物，使用的抗体是Akt (pan) (40D4) Mouse mAb鼠单抗 (Biotinylated)。 | |
Flow cytometric analysis of Jurkat cells using Akt (pan) (40D4) Mouse mAb (Biotinylated) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097 (red). 应用Akt (pan) (40D4) Mouse mAb鼠单抗 (Biotinylated)（蓝色）流式细胞术分析Jurkat细胞，并和Mouse (MOPC-21) mAb IgG1同型对照(Biotinylated) #4097 (红色）比较。 | |
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2) using Akt (pan) (40D4) Mouse mAb (Biotinylated) and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097. Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blot analysis was performed using Akt (pan) (C67E7) Rabbit mAb #4691. |
