| 货号 | 9602S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 4 hr; right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 Phalloidin #8878 (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. 共聚焦免疫荧光分析HeLa细胞，未处理（左）和 Staurosporine #9953（1 μM, 4 hr；右）处理，使用抗体为Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗(Alexa Fluor® 647 Conjugate)（蓝色伪彩）。Alexa Fluor® 488 Phalloidin #8878（绿色）标记肌动蛋白丝。红色= Propidium Iodide (PI)/RNase Staining Solution #4087. | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide #2200 (green), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate). 流式细胞术检测Jurkat细胞，未处理（蓝）和 etoposide #2200 处理（绿），使用抗体为Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate)兔单抗。 |
