Cyclin B1 (V152) Mouse mAb (Alexa Fluor® 647 Conjugate)
货号：
4118S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 4118S |
| 描述 | Cell Signaling Technology Antibody conjugated to Alexa Fluor®647 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human cells. The unconjugated antibody #4135 reacts with, among others, human, mouse and hamster Cyclin B1. CST expects that Cyclin B1 Mouse mAb (Alexa Fluor®647 conjugate) will also recognize Cyclin B1 in these species.该抗体偶联于Alexa Fluor®647 荧光染料，并经过内部测试，直接进行人类细胞的流式细胞术分析。货号为#4135，未偶联荧光染料的Cyclin B1抗体可以识别人、小鼠和仓鼠周期蛋白B1。CST期望 Cyclin B1 Mouse mAb (Alexa Fluor®647 conjugate) 同样可以识别以上物种的周期蛋白B1。 |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | F |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13).cdc2蛋白激酶的激活参与调节所有的真核细胞进入有丝分裂。cdc2的激活受多个步骤的调控，包括周期蛋白B的结合、cdc2苏氨酸161位的磷酸化和苏氨酸14位和15位的去磷酸化(1-5)。CDK抑制剂和CDK相关的细胞周期蛋白的水平可通过磷酸化、泛素化和降解来调节，允许了细胞周期事件的化学计量调节(6)。四个周期蛋白B1磷酸化位点(丝氨酸126位，128位，133位和147位)位于胞质滞留信号(CRS)结构域，并可以调节G2/S检验点周期蛋白B1的核迁移(8-10)。周期蛋白B1的磷酸化参与了cdc25C依赖的cdc2 酪氨酸15位的去磷酸化和随后的cdc2/周期蛋白B1激活(11)。尽管cdc2/周期蛋白B1本身能够磷酸化丝氨酸126位和128位(8)，polo样蛋白激酶优先磷酸化丝氨酸133位，也可能磷酸化丝氨酸147位(11-13)。 |
| 存放说明 | 4C |
参考图片Two-color flow cytometric analysis of asynchronous Jurkat cells, using Cyclin B1 (V152) Alexa Fluor® 647 Conjugate and Phospho-Histone H3 (Ser10) Alexa Fluor® 488 Conjugate #9708. Cells represented in green are positive for cyclin B1 and phospho-histone H3, while cells represented in blue are positive for phospho-histone H3 and negative for cyclin B1. Both cell populations (green and blue) correspond to cells undergoing mitosis.双色流式细胞术分析异步Jurkat细胞，使用的抗体为Cyclin B1 (V152) Alexa Fluor® 647 Conjugate 和 Phospho-Histone H3 (Ser10) Alexa Fluor® 488 Conjugate #9708。绿色显示细胞周期蛋白B1和磷酸化组蛋白H3阳性的细胞，蓝色显示磷酸化组蛋白H3阳性但细胞周期蛋白B1阴性的细胞。两个细胞群（绿色和蓝色）都处于有丝分裂期。 |
