| 货号 | 3199S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated E-Cadherin (24E10) Rabbit mAb #3195.抗体与Alexa Fluor®488荧光染料偶联，并经过内部测试，由人类细胞直接流式细胞仪分析。抗体被认为与未偶联的E-Cadherin (24E10)兔源单抗#3195表现同样的物种交叉反应。 |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | IF-P/IF-IC/F |
| 目标/特异性 | E-Cadherin (24E10) Rabbit mAb detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. |
| 使用方法 | F(1:50) IF-IC (1:800) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of paraffin-embedded human breast carcinoma using E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).用 E-Cadherin (24E10)兔单抗（Alexa Fluor® 488标记）（绿色）对石蜡包埋的人乳腺癌组织进行进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5®（DNA荧光染料）。 | |
Confocal immunofluorescent analysis of MCF7 cells using E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488 Conjugate)(green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).使用 E-Cadherin (24E10)兔单抗（Alexa Fluor® 488标记）（绿色）对MCF7细胞进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5® （DNA荧光染料）。 | |
Flow cytometric analysis of HeLa (blue) and MCF7 cells (green) using E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488 Conjugate).使用E-Cadherin (24E10) 兔单抗（Alexa Fluor® 488标记）对HeLa（蓝色）和MCF7细胞（绿色）进行流式细胞分析。 |
