E-Cadherin (24E10) Rabbit mAb (PE Conjugate)
货号：
7559S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 7559S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated E-Cadherin (24E10) Rabbit mAb #3195. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | E-Cadherin (24E10) Rabbit mAb (PE Conjugate) detects endogenous levels of total E-cadherin protein. This antibody does not cross-react with related family members, such as N-cadherin. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HeLa (blue) and MCF7 (green) cells using E-Cadherin (24E10) Rabbit mAb (PE Conjugate). 流式细胞术分析HeLa (蓝色) 和MCF7 (绿色)细胞，使用的抗体是E-Cadherin (24E10) Rabbit mAb (PE Conjugate)。 |
