| 货号 | 3906S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GAPDH (14C10) Rabbit mAb #2118. |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | GAPDH (14C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total GAPDH protein. |
| 使用方法 | F(1:50) IF-IC (1:800) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimers diseases (4,5). |
| 存放说明 | 4C |
Flow cytometric analysis of HeLa cells using GAPDH (14C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) compared to Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red). 流式细胞仪检测Hela细胞，所用抗体为GAPDH (14C10) Rabbit mAb兔单抗 (Alexa Fluor® 488 Conjugate) (绿)，对照为Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 488 Conjugate)#2975 (红). | |
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (14C10) (Alexa Fluor® 488 Conjugate) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测 C2C12，所用抗体为APDH (14C10) (Alexa Fluor® 488 Conjugate) Rabbit mAb兔单抗 (绿)，蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084)。 |
