GAPDH (14C10) Rabbit mAb (Alexa Fluor® 647 Conjugate)
货号：
3907S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 3907S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (GAPDH (14C10) Rabbit mAb #2118). |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | GAPDH (14C10) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total GAPDH protein. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm. |
| 使用方法 | F(1:50)
IF-IC (1:400) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimers diseases (4,5). |
| 存放说明 | 4C |
参考图片 |
Flow cytometric analysis of HeLa cells using GAPDH (14C10) Rabbit mAb (Alexa Fluor® 647 Conjugate) (green) compared to Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (red). |
