| 货号 | 5014S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated GAPDH (14C10) Rabbit mAb #2118 reacts with human, mouse, rat and monkey GAPDH. CST expects that GAPDH (14C10) Rabbit mAb (Biotinylated) will also recognize GAPDH in these species. |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | GAPDH (14C10) Rabbit mAb (Biotinylated) detects endogenous levels of total GAPDH protein. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:150) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3) and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimers diseases (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using GAPDH (14C10) Rabbit mAb (Biotinylated). Western blot检测HeLa, NIH/3T3 and C6 细胞提取物，所用抗体为GAPDH (14C10) Rabbit mAb兔单抗(Biotinylated). | |
Flow cytometric analysis of HeLa cells, using GAPDH (14C10) Rabbit mAb (Biotinylated) (blue) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Biotinylated) #4096 (red). 流式细胞检测HeLa细胞，所用抗体为GAPDH (14C10) Rabbit mAb兔单抗 (Biotinylated) (绿) 和XP® Rabbit (DA1E) mAb IgG Isotype Control (Biotinylated) #4096 (红). | |
Confocal immunofluorescent analysis of MCF-7 cells using GAPDH (14C10) Rabbit mAb (Biotinylated) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测 MCF-7细胞提取物，所用抗体为GAPDH (14C10) Rabbit mAb兔单抗 (Biotinylated) (red). ，蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084) |
