| 货号 | 9016S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat cells and flow cytometry in human and mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Insulin (C27C9) Rabbit mAb #3014. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | IF-F/F |
| 目标/特异性 | Insulin (C27C9) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total insulin protein. |
| 使用方法 | F(1:50) IF-F (1:200) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2). |
| 存放说明 | 4C |
Flow cytometric analysis of HeLa (blue) and β-TC-6 (green) cells using Insulin (C27C9) Rabbit mAb (Alexa Fluor® 488 Conjugate). 流式细胞仪检测HeLa (蓝)和beta-TC-6 (绿)细胞，使用的抗体为Insulin (C27C9) Rabbit mAb兔单抗 ( Alexa Fluor® 488 Conjugate)。 | |
Confocal immunofluorescent analysis of rat pancreas using Insulin (C27C9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠胰腺，使用抗体为Insulin (C27C9) Rabbit mAb兔单抗 (Alexa Fluor® 488 Conjugate)（绿色）。DY-554 phalloidin（红色）标记肌动蛋白微丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 |
