IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor® 488 Conjugate)
货号：
5743S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 5743S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814. |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig/GuineaPig |
| 来源宿主 | Mouse |
| 应用 | F |
| 目标/特异性 | IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor® 488 Conjugate) detects endogenous levels of total IκBα protein. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HeLa cells, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor® 488 Conjugate) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).流式细胞仪研究 HeLa细胞，所用抗体为IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor® 488 Conjugate)(蓝色)与 Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878(红色)相对比。 |
