| 货号 | 8617S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Lamin A/C (4C11) Mouse mAb #4777. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | IF-F/IF-IC/F |
| 目标/特异性 | Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of lamin A and C proteins. It also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2. |
| 使用方法 | F(1:50) IF-F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6). |
| 存放说明 | 4C |
Flow cytometric analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red). 流式细胞术分析 Hela 细胞，所用抗体为Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (绿色) 和浓度相匹配的Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (红色)。 | |
Confocal immunofluorescent analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). 共聚焦免疫荧光分析Hela细胞，所用抗体为Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) 鼠单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。 | |
Confocal immunofluorescent analysis of normal rat brain using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye). 共聚焦免疫荧光分析正常的大鼠脑组织，所用抗体为Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) 鼠单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。 Blue pseudocolor = DRAQ5® (DNA 荧光染料)。 |
