mTOR (7C10) Rabbit mAb (Alexa Fluor® 488 Conjugate)
货号：
5043S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 5043S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated mTOR (7C10) Rabbit mAb #2983. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | mTOR (7C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total mTOR protein. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HeLa cells using mTOR (7C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red). 流式细胞术分析HeLa 细胞的提取物，所用抗体为mTOR (7C10) Rabbit mAb (Alexa Fluor ® 488 Conjugate) 兔单抗(蓝色) 与Rabbit (DA1E) mAb IgG XP™ Isotype Control (Alexa Fluor ® 488 Conjugate) #2975 (红色)作对比。 |
